| 质粒名称: | pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 35499 |
| 存储实验室: | Karl Deisseroth |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5385 bp |
| 完整质粒大小: | 7203 bp |
| 载体修饰: | Addition of a CaMKIIa promoter and a WPRE |
| 载体类型: | AAV哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ChR1-VChR1 Chimera |
| 物种来源: | Synthetic |
| 插入大小: | 1818 bp |
| 基因突变: | E122T and E162T |
| GenBank编号 | AEL28923.1 |
| 启动子 | CMV |
| 标签/融合蛋白: | EYFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | AGTCCTGCAGTATTGTGTAT |
| 3' 测序引物 | GCAATAGCATGATACAAAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP was a gift from Karl Deisseroth (爱迪基因 plasmid # 35499) |
| 参考文献部分: | Neocortical excitation/inhibition balance in information processing and social dysfunction. Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K. Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. 10.1038/nature10360 PubMed 21796121 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-TS-EYFP质粒图谱.png)
载体简介
载体序列
LOCUS pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP 7193 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7193)
AUTHORS Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ,
Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R,
Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
TITLE Neocortical excitation/inhibition balance in information processing
and social dysfunction.
JOURNAL Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
PUBMED 21796121
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP|
FEATURES Location/Qualifiers
source 1..7193
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 55..184
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
CDS 2633..3349
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(2675..2696)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2936..2955)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3283..3304
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3374..3962
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3427..3447)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3845..3856)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3994..4470
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(4193..4212)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 4510..4650
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 4510..4639
/label=AAV2 ITR
rep_origin 4725..5180
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4812..4831)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5022..5043
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5197..5216)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5316..5338
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5376..5394)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5462..5566
/gene="bla"
/label=AmpR promoter
CDS 5567..6427
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5785..5804)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6598..7186
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7087..7106
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca
61 ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc
121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
181 tcctgcggcc gcacgcgttt aacattatgg ccttaggtca cttcatctcc atggggttct
241 tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca
301 gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca
361 tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc
421 aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg
481 ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg
541 aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg
601 gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg
661 acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta
721 cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct
781 ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca
841 aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg
901 taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag
961 atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac
1021 ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta
1081 aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa
1141 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca
1201 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga
1261 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
1321 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
1381 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg
1441 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag ctctagagcg
1501 gtaccggatc cccgggtacc ggtaggccac catgtcgcgg aggccatggc ttcttgccct
1561 agcgctggca gtggcgctgg cggccggcag cgcaggagcc tcgactggca gtgacgcgac
1621 ggtgccggtc gcgactcagg atggccccga ctacgttttc caccgtgccc acgagcgcat
1681 gctcttccaa acctcataca ctcttgagaa caatggttct gttatttgca tcccgaacaa
1741 cggccagtgc ttctgcttgg cttggcttaa atccaacgga acaaatgccg agaagttggc
1801 tgccaacatt ctgcagtgga ttacttttgc gctttcagcg ctctgcctga tgttctacgg
1861 ctaccagacc tggaagtcta cttgcggctg ggagaccatt tacgtggcca cgatcgagat
1921 gatcaagttc atcatcgagt atttccatga gtttgacgaa cctgcggtga tctactcatc
1981 caacggcaac aagaccgtgt ggcttcgtta cgcgacctgg ctgctcacct gcccggtcct
2041 tctgatccat ctgagcaacc tcacaggcct gaaggacgat tatagcaaaa ggactatggg
2101 cctgttggtt tctgatgtgg gatgcatcgt gtggggcgca accagcgcca tgtgtacggg
2161 gtggacgaag atcctgttct tcctcatctc attgagctat ggtatgtata cctattttca
2221 tgctgctaaa gtttatatcg aagcattcca cacagttcca aaagggattt gtcgagaact
2281 ggtccgagtg atggcctgga cattctttgt ggcttgggga atgtttccag tcctgtttct
2341 gctgggcacg gaaggattcg gtcatatcag cccttatgga tctgccattg ggcactccat
2401 cctcgacctg attgcaaaga acatgtgggg tgtgctgggg aattacctgc gcgtcaaaat
2461 ccacgagcac atcctgttgt atggcgacat cagaaagaag cagaaaatta cgatcgccgg
2521 ccaagagatg gaggttgaga cactggtggc tgaagaggag gacgcggccg ccaagagcag
2581 gatcaccagc gagggcgagt acatccccct ggaccagatc gacatcaacg tggtgagcaa
2641 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
2701 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
2761 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
2821 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
2881 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
2941 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
3001 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
3061 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
3121 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
3181 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
3241 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
3301 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat
3361 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
3421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
3481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
3541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
3601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg gaactttcgc
3661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
3721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
3781 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
3841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
3901 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
3961 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
4021 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
4081 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
4141 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
4201 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
4261 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
4321 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
4381 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
4441 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
4501 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4561 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4621 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4681 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4801 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4921 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5041 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
5101 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
5161 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6721 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg cca
//
